ABSTRACT
<p><b>OBJECTIVE</b>The aim of this study was to investigate the expression and significance of P311 and ITGB4BP in non-small cell lung cancer (NSCLC).</p><p><b>METHODS</b>Tissue microarrays were prepared from 80 NSCLC specimens and examined by immunohistochemistry.</p><p><b>RESULTS</b>The positive rates of P311 and ITGB4BP expression were 77.5% (62/80) and 82.5% (66/80), respectively. The double positive expression rate was 73.8% (59/80). The consistency rate was 87.5%, and there was a significant consistency between P311 and ITGB4BP expressions (Kappa = 0.611, P < 0.001).</p><p><b>CONCLUSION</b>There may be a new signaling pathway P311-ITGB4BP in NSCLC, and it may regulate the lung cancer cell migration.</p>
Subject(s)
Humans , Adenocarcinoma , Metabolism , Carcinoma, Non-Small-Cell Lung , Metabolism , Carcinoma, Squamous Cell , Metabolism , Eukaryotic Initiation Factors , Metabolism , Immunohistochemistry , Lung Neoplasms , Metabolism , Nerve Tissue Proteins , Metabolism , Oncogene Proteins , Metabolism , Paraffin Embedding , Signal Transduction , Tissue Array AnalysisABSTRACT
<p><b>OBJECTIVE</b>To analyze the relationship between malignant transformation and abnormal expression of eukaryotic initiation factor 3 (eIF3 p36) in human bronchial epithelial (16HBE) cells induced by cadmium chloride (CdCl2).</p><p><b>METHODS</b>16HBE cells were treated several times with different concentrations of CdCl2. Tumorigenic potential of transformed cells was identified by assays for anchorage-independent growth in soft agar and for tumorigenicity in nude mice after the 35th passage. Total RNA was isolated from 16HBE cells induced by CdCl2, including non-transformed, Cd-transformed, and Cd-tumorigenic cell lines. Special primers for eIF3 p36 were designed and the expression of eIF3 mRNA in different cell lines was detected with fluorescent quantitative-polymerase chain reaction technique (FQ-PCR).</p><p><b>RESULTS</b>The 35th passage of 16HBE cells transformed by CdCl2 exhibited overlapping growth. Compared with the non-transformed cells, colonies of transformed cell lines in soft agar showed statistically significant increases and dose-dependent effects (P<0.01). All Cd-induced transformed cell lines formed tumors in nude mice within 2 weeks of inoculation, but none of the mice injected with non-transformed cells showed tumors even after 3 weeks. All tumors were pathologically identified as poorly differentiated squamous cell carcinoma. The eIF3 p36 genes in different stages of 16HBE cells transformed by CdCl2 were elevated as compared with the non-transformed control (P<0.01), and the eIF3 expression increased with the degree of cell malignancy.</p><p><b>CONCLUSION</b>CdCl2 is capable of inducing morphological transformation in 16HBE cells and transformed cells are potentially tumorigenic. Over-expression of eIF3 p36 is positively correlated with malignant transformation of 16HBE cells induced by CdCl2 and may be one of the molecular mechanisms potentially responsible for carcinogenesis due to Cd.</p>
Subject(s)
Animals , Humans , Mice , Base Sequence , Bronchi , Cell Biology , Metabolism , Cadmium Chloride , Pharmacology , Cell Transformation, Neoplastic , DNA Primers , Epithelial Cells , Metabolism , Eukaryotic Initiation Factors , Metabolism , Mice, Nude , Polymerase Chain ReactionABSTRACT
The presence of antisense transcripts has been described in a wide variety of eucaryotic organisms, with a diversity of implied biological functions in development, control of cell cycles, hormonal responses, control of proliferation, structure, viral replication, and others. Reports have suggested that this type of control may operate at different levels of genic expression, whether in transcription, maturation, transport, stability, or translation of a given trascript. It is evident that the expression of antisense RNA has true importance in Eukaryotes, as already established in prokaryotic organisms. The objective of the present review is to present the advances in the mechanics of regulation, and roles already established for the expressions of antisense RNA in eukaryotic organisms.
La presencia de transcritos antisentidos ha sido descrita en una amplia variedad de organismos eucariontes, implicados en una diversidad de funciones biológicas como el desarrollo, el control del ciclo celular, respuesta hormonal, control de la proliferación, estructura, replicación viral, etc. Los informes indican que este control puede darse a diversos niveles de la expresión génica, ya sea en la transcripción, maduración, transporte, estabilidad y traducción de un determinado transcrito. Es evidente que la expresión del ARN antisentido tiene una real importancia en eucariontes, como ya ha sido establecido en organismos procariontes. Esta revisión tiene como objetivo mostrar los avances en los mecanismos de regulación y roles ya establecidos de la expresión de ARN antisentidos en organismos eucariontes.
Subject(s)
Humans , Eukaryotic Initiation Factors/genetics , Oligoribonucleotides, Antisense , RNAABSTRACT
Ribosome recruitment to eukaryotic mRNAs is generally thought to occur by a scanning mechanism, whereby the 40S ribosomal subunit binds in the vicinity of the 5'cap structure of the mRNA and scans until an AUG codon is encountered in an appropriate sequence context. Study of the picornaviruses allowed the characterization of an alternative mechanism of translation initiation. Picornaviruses can initiate translation via an internal ribosome entry segment (IRES), an RNA structure that directly recruits the 40S ribosomal subunits in a cap and 5' end independent fashion. Since its discovery, the notion of IRESs has extended to a number of different virus families and cellular RNAs. This review summarizes features of both cap-dependent and IRES-dependent mechanisms of translation initiation and discusses the role of cis-acting elements, which include the 5'cap, the 5'-untranslated region (UTR) and the poly(A) tail as well as the possible roles of IRESs as part of a cellular stress response mechanism and in the virus replication cycle.
Subject(s)
Humans , Animals , RNA, Messenger/analysis , RNA, Messenger/biosynthesis , RNA, Messenger/genetics , Eukaryotic Cells/cytology , Eukaryotic Cells/physiology , Eukaryotic Cells/virology , Eukaryotic Initiation Factors/analysis , Eukaryotic Initiation Factors/biosynthesis , Eukaryotic Initiation Factors/genetics , Proteins , RNA, Ribosomal/analysis , RNA, Ribosomal/biosynthesis , RNA, Ribosomal/chemical synthesisABSTRACT
An efficient (12 steps, 12% overallyield) and stereoselective total synthesis of (±)-serricornine (1) the sex pheromone of the cigarette beetle (Lasioderma serricornine F) is described. The preparation of intermediate 5, which encompasses the proper relative configuration of three contiguous chiral centers of (±)-invictolide, (3), is discussed.